Molecular detection of hepatitis E virus in sewage samples

Appl Environ Microbiol. 2010 Sep;76(17):5870-3. doi: 10.1128/AEM.00336-10. Epub 2010 Jul 2.

Abstract

Human hepatitis E virus (HEV) is considered an emerging pathogen in industrialized countries. In Italy, the true burden of HEV infection is unknown. Molecular HEV screening of raw sewage samples from 11 wastewater treatment plants yielded 19 positives (16%; 18 genotype I, 1 genotype III) evenly distributed throughout Italy. Evidence that HEV could be establishing itself in our region is accumulating and may justify more active surveillance to monitor its spread.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • DNA Primers / genetics
  • Genotype
  • Hepatitis E virus / genetics*
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Italy
  • Molecular Sequence Data
  • RNA, Viral / genetics*
  • RNA, Viral / isolation & purification*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Sequence Homology
  • Sewage / virology*
  • Virology / methods*

Substances

  • DNA Primers
  • RNA, Viral
  • Sewage

Associated data

  • GENBANK/FN796464
  • GENBANK/FN796465
  • GENBANK/FN796466
  • GENBANK/FN796467
  • GENBANK/FN796468
  • GENBANK/FN796469
  • GENBANK/FN796470
  • GENBANK/FN796471
  • GENBANK/FN796472
  • GENBANK/FN796473
  • GENBANK/FN796474
  • GENBANK/FN796475
  • GENBANK/FN796476
  • GENBANK/FN796477
  • GENBANK/FN796478
  • GENBANK/FN796479
  • GENBANK/FN796480
  • GENBANK/FN796481