Hydrogen peroxide vapor decontamination of an intensive care unit to remove environmental reservoirs of multidrug-resistant gram-negative rods during an outbreak

Am J Infect Control. 2010 Nov;38(9):754-6. doi: 10.1016/j.ajic.2010.03.010.


Multidrug-resistant gram-negative rods (MDR-GNR) are an increasing cause for concern in intensive care units (ICUs). We used hydrogen peroxide vapor (HPV) to decontaminate our entire ICU in an attempt to eradicate undetected environmental contamination during outbreaks of MDR-GNR. Surface sampling identified GNR, including MDR strains, on 10 (48%) of 21 areas cultured after intensive cleaning but before decontamination with HPV, and on no areas after HPV. No new cases of Acinetobacter were identified for approximately 3 months after HPV.

MeSH terms

  • Cross Infection / epidemiology*
  • Decontamination / methods*
  • Disinfectants / pharmacology
  • Disinfection / methods*
  • Drug Resistance, Multiple, Bacterial*
  • Environmental Microbiology
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / isolation & purification
  • Gram-Negative Bacterial Infections / epidemiology*
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Intensive Care Units
  • Volatilization


  • Disinfectants
  • Hydrogen Peroxide