Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria

FEBS Lett. 1991 Jun 17;284(1):51-6. doi: 10.1016/0014-5793(91)80759-v.

Abstract

We have determined the N-termini of 26 proteins of the large ribosomal subunit from yeast mitochondria by direct amino acid micro-sequencing. The N-terminal sequences of proteins YmL33 and YmL38 showed a significant similarity to eubacterial ribosomal (r-) proteins L30 and L14, respectively. In addition, several proteins could be assigned to their corresponding yeast nuclear genes. Based on a comparison of the protein sequences deduced from the corresponding DNA regions with the N-termini of the mature proteins, the putative leader peptides responsible for mitochondrial matrix-targeting were compiled. In most leader sequences a relative abundance of aromatic amino acids, preferentially phenylalanine, was found.

MeSH terms

  • Amino Acid Sequence
  • Electrophoresis, Gel, Two-Dimensional
  • Fungal Proteins / chemistry*
  • Mitochondria / metabolism*
  • Molecular Sequence Data
  • Ribosomal Proteins / chemistry*
  • Saccharomyces cerevisiae / metabolism*
  • Sequence Alignment

Substances

  • Fungal Proteins
  • Ribosomal Proteins