Cloning of nocardioform DNA conferring the ability to inactivate rifampicin

FEMS Microbiol Lett. 1991 Apr 15;63(2-3):247-50. doi: 10.1016/0378-1097(91)90093-p.


A novel mechanism of resistance to rifampicin has recently been reported in which this antibiotic is inactivated. Here we describe the cloning of DNA from a nocardiofrom strain conferring this ability to inactivate rifampicin. Cloning was on the basis of complementation, as an increased resistance to the antibiotic. The genetic information was on a 8.3-kb BglII fragment.

MeSH terms

  • Cloning, Molecular*
  • DNA, Bacterial / genetics*
  • Drug Resistance, Microbial / genetics*
  • Escherichia coli / genetics
  • Mutation
  • Plasmids
  • Restriction Mapping
  • Rhodococcus / drug effects
  • Rhodococcus / genetics*
  • Rifampin / antagonists & inhibitors*
  • Rifampin / pharmacology
  • Transformation, Bacterial


  • DNA, Bacterial
  • Rifampin