[Analysis of mutations in DNA polymerase and thymidine kinase genes of herpes simplex virus clinical isolates resistant to antiherpetic drugs]

Mol Biol (Mosk). May-Jun 2010;44(3):488-96. doi: 10.1134/s0026893310030192.
[Article in Russian]

Abstract

The primary structures of DNA-polymerase (ul30) and thymidine kinase (ul23) genes from several herpes simplex virus type 1 (HSV-1) clinical isolates which differed in sensitivity for a number of antiherpetic drugs were determined and compared with those for two laboratory HSV-1 strains one of which was ACV-sensitive (L2), while the another was resistant (L2) to ACV. The phylogenetic analysis of the sequences showed that conserved regions of ul30 gene of HSV-1 clinical isolates and L2 strain were homologous with the exception of point mutations and degenerated substitutions. Several new mutations in the HSV-1 DNA-polymerase and thymidine kinase functional domains were established and identified as the substitutions associated with the strain-resistance to ACV and other drugs.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology*
  • Antiviral Agents / pharmacology*
  • Cell Line
  • DNA-Directed DNA Polymerase / genetics*
  • DNA-Directed DNA Polymerase / metabolism
  • Drug Resistance, Viral / genetics*
  • Exodeoxyribonucleases / genetics*
  • Exodeoxyribonucleases / metabolism
  • Herpes Simplex / drug therapy
  • Herpes Simplex / enzymology
  • Herpes Simplex / genetics*
  • Herpesvirus 1, Human / enzymology
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / isolation & purification
  • Humans
  • Point Mutation*
  • Thymidine Kinase / genetics*
  • Thymidine Kinase / metabolism
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Antiviral Agents
  • Viral Proteins
  • Thymidine Kinase
  • DNA-Directed DNA Polymerase
  • Exodeoxyribonucleases
  • DNA polymerase, Simplexvirus
  • Acyclovir