Ankyrin-B regulates Kir6.2 membrane expression and function in heart

J Biol Chem. 2010 Sep 10;285(37):28723-30. doi: 10.1074/jbc.M110.147868. Epub 2010 Jul 7.


Ankyrin polypeptides are critical for normal membrane protein expression in diverse cell types, including neurons, myocytes, epithelia, and erythrocytes. Ankyrin dysfunction results in defects in membrane expression of ankyrin-binding partners (including ion channels, transporters, and cell adhesion molecules), resulting in aberrant cellular function and disease. Here, we identify a new role for ankyrin-B in cardiac cell biology. We demonstrate that cardiac sarcolemmal K(ATP) channels directly associate with ankyrin-B in heart via the K(ATP) channel alpha-subunit Kir6.2. We demonstrate that primary myocytes lacking ankyrin-B display defects in Kir6.2 protein expression, membrane expression, and function. Moreover, we demonstrate a secondary role for ankyrin-B in regulating K(ATP) channel gating. Finally, we demonstrate that ankyrin-B forms a membrane complex with K(ATP) channels and the cardiac Na/K-ATPase, a second key membrane transporter involved in the cardiac ischemia response. Collectively, our new findings define a new role for cardiac ankyrin polypeptides in regulation of ion channel membrane expression in heart.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ankyrins / genetics
  • Ankyrins / metabolism*
  • Cell Line
  • Cell Membrane / genetics
  • Cell Membrane / metabolism*
  • Gene Expression Regulation*
  • Humans
  • Ion Channel Gating / physiology
  • Mice
  • Mice, Knockout
  • Myocardial Ischemia / genetics
  • Myocardial Ischemia / metabolism
  • Myocardium / metabolism*
  • Myocytes, Cardiac / metabolism
  • Potassium Channels, Inwardly Rectifying / biosynthesis*
  • Potassium Channels, Inwardly Rectifying / blood*
  • Sarcolemma / genetics
  • Sarcolemma / metabolism


  • Ank2 protein, mouse
  • Ankyrins
  • Kir6.2 channel
  • Potassium Channels, Inwardly Rectifying