A strategy for direct mapping and identification of mutations by whole-genome sequencing

Genetics. 2010 Sep;186(1):427-30. doi: 10.1534/genetics.110.119230. Epub 2010 Jul 6.

Abstract

Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Mapping / methods*
  • DNA Mutational Analysis / methods*
  • Genome, Bacterial / genetics*
  • Genomics
  • Mutagenesis
  • Mutation*