Quantitative genetic variation of esterase activity associated with a gene amplification in Culex quinquefasciatus

Heredity (Edinb). 1991 Apr;66 ( Pt 2):265-72. doi: 10.1038/hdy.1991.33.

Abstract

Amplification of the esterase B1 gene is responsible for insecticide resistance in the mosquito Culex quinquefasciatus. We used a mating scheme to isolate chromosomes carrying amplified esterase genes from a long-selected laboratory strain (Tem-R) to determine whether observed variation in esterase activity had a genetic basis. The amplified esterase genes segregated as a block and a possible newly arisen esterase B1 copy-number variant was found among the progeny of females which carried amplified B1 genes on only one homologue. A quantitative genetic analysis found significant genetic variation of esterase activity among families which carried different amplification-bearing chromosomes from the Tem-R strain. Esterase B1 copy-number variation among these Tem-R chromosomes is the most likely basis for the observed genetic variation in esterase activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Crosses, Genetic
  • Culex / genetics*
  • Female
  • Gene Amplification
  • Insecticide Resistance / genetics
  • Male
  • Multigene Family*
  • Proteins / analysis
  • Regression Analysis
  • Sex Factors

Substances

  • Proteins