Hair Cell Damage and Recovery Following Chronic Application of Kanamycin in the Chick Cochlea

Hear Res. 1991 Apr;52(2):356-68. doi: 10.1016/0378-5955(91)90025-5.

Abstract

Three-day old chicks were given kanamycin at a dose of 200 mg/kg/day for 10 days and their cochleae were processed for scanning electron microscopy at 1, 3, 7 and 14 days following the last injection. Both hair cells and supporting cells were damaged by kanamycin in the basal 35% of the basilar papilla. By 14 days post-treatment, however, most of the damaged region had been replaced with regenerating hair cells and supporting cells. The base-to-apex gradient of morphological development along the cochlea was observed in the process of regeneration. Kinocilium and microvilli were observed on the apical surfaces of the regenerating hair cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chickens
  • Cilia / drug effects
  • Cilia / ultrastructure
  • Cochlea / drug effects*
  • Cochlea / physiology
  • Cochlea / ultrastructure
  • Hair Cells, Auditory / drug effects*
  • Hair Cells, Auditory / physiology
  • Hair Cells, Auditory / ultrastructure
  • Kanamycin / administration & dosage
  • Kanamycin / toxicity*
  • Male
  • Microscopy, Electron, Scanning
  • Regeneration
  • Time Factors

Substances

  • Kanamycin