Multiphoton microscopy with near infrared contrast agents

J Biomed Opt. 2010 May-Jun;15(3):030505. doi: 10.1117/1.3420209.


While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cells, Cultured
  • Contrast Media / chemistry*
  • Epithelial Cells / chemistry
  • Fluorescent Dyes / chemistry*
  • Humans
  • Immunohistochemistry
  • Lasers
  • Microscopy, Fluorescence, Multiphoton / instrumentation
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Neoplasms / diagnosis
  • Spectroscopy, Near-Infrared / instrumentation
  • Spectroscopy, Near-Infrared / methods*


  • Contrast Media
  • Fluorescent Dyes