An easy-to-use tool for planning and modeling a calorimetric titration

Anal Biochem. 2010 Nov 1;406(1):91-3. doi: 10.1016/j.ab.2010.06.050. Epub 2010 Jul 6.


Isothermal titration calorimetry (ITC) is widely employed to measure thermodynamic properties of binding interactions between two macromolecules or a macromolecule and a small ligand. No labeling of interacting species is required for ITC, but this advantage is offset by potentially material-consuming experimental optimization complicated by an indirect readout of an ITC titration. Here we present a simple, practical, and portable spreadsheet-based tool for planning and modeling an ITC titration experiment accompanied by basic guidelines.

MeSH terms

  • Calorimetry / methods*
  • Models, Theoretical*
  • Software
  • Statistics as Topic / methods*