A genomic diagnostic tool for human endometrial receptivity based on the transcriptomic signature

Fertil Steril. 2011 Jan;95(1):50-60, 60.e1-15. doi: 10.1016/j.fertnstert.2010.04.063. Epub 2010 Jul 8.


Objective: To create a genomic tool composed of a customized microarray and a bioinformatic predictor for endometrial dating and to detect pathologies of endometrial origin. To define the transcriptomic signature of human endometrial receptivity.

Design: Two cohorts of endometrial samples along the menstrual cycle were used: one to select the genes to be included in the customized microarray (endometrial receptivity array [ERA]), and the other to be analyzed by ERA to train the predictor for endometrial dating and to define the transcriptomic signature. A third cohort including pathological endometrial samples was used to train the predictor for pathological classification.

Setting: Healthy oocyte donors and patients.

Patient(s): Healthy fertile women (88) and women with implantation failure (5) or hydrosalpinx (2).

Intervention(s): Human endometrial biopsies.

Main outcome measure(s): The gene expression of endometrial biopsies.

Result(s): The ERA included 238 selected genes. The transcriptomic signature was defined by 134 genes. The predictor showed a specificity of 0.8857 and sensitivity of 0.99758 for endometrial dating, and a specificity of 0.1571 and a sensitivity of 0.995 for the pathological classification.

Conclusion(s): This diagnostic tool can be used clinically in reproductive medicine and gynecology. The transcriptomic signature is a potential endometrial receptivity biomarkers cluster.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Cluster Analysis
  • Endometrium / physiology*
  • Female
  • Gene Expression Profiling / methods*
  • Gene Expression Profiling / standards
  • Genomics / methods*
  • Genomics / standards
  • Humans
  • Infertility, Female / diagnosis
  • Infertility, Female / genetics
  • Menstrual Cycle / physiology
  • Predictive Value of Tests
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / standards
  • Sensitivity and Specificity
  • Uterine Diseases / diagnosis*
  • Uterine Diseases / genetics*