Genome-wide chromatin maps derived from limited numbers of hematopoietic progenitors

Nat Methods. 2010 Aug;7(8):615-8. doi: 10.1038/nmeth.1478. Epub 2010 Jul 11.


Current methods for whole-genome mapping of protein-DNA interactions, performed by coupling chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), require large amounts of starting materials, which precludes their application to rare cell types. Here we combine a high-sensitivity ChIP assay with a new library preparation procedure to map histone modifications in as few as 10,000 cells. We used the technique to characterize mouse hematopoietic progenitors and thereby gain insight into their developmental program.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatin Immunoprecipitation
  • Chromosome Mapping / methods*
  • DNA-Binding Proteins / genetics*
  • Gene Library
  • Hematopoietic Stem Cells*
  • Histones / metabolism
  • Mice
  • Sequence Analysis, DNA


  • DNA-Binding Proteins
  • Histones

Associated data

  • GEO/GSE22075