Odorant receptor from the southern house mosquito narrowly tuned to the oviposition attractant skatole

J Chem Ecol. 2010 Aug;36(8):797-800. doi: 10.1007/s10886-010-9828-9. Epub 2010 Jul 11.

Abstract

Oviposition attractants are environmental cues that allow Culex gravid female mosquitoes to locate suitable sites for egg-laying and, therefore, may be exploited for environmentally friendly strategies for controlling mosquito populations. Naturally occurring skatole has been identified as an oviposition attractant for the Southern House mosquito, Culex quinquefasciatus. Previously, we identified in Cx. quinquefasciatus female antennae an olfactory receptor neuron (ORN) highly sensitive to skatole and an odorant-binding protein involved in the detection of this semiochemical. Here, we describe the characterization of an odorant receptor (OR), CquiOR10, which is narrowly tuned to skatole when expressed in the Xenopus oocyte system. Odorant-induced response profiles generated by heterologously expressed CquiOR10 suggest that this OR is expressed in the mosquito ORN sensitive to skatole. However, geranylacetone, which stimulates the antennal ORN, was not detected by CquiOR10-expressing oocytes, thus raising interesting questions about reception of oviposition attractants in mosquitoes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cues
  • Culex / drug effects*
  • Culex / metabolism*
  • Culex / physiology
  • Dose-Response Relationship, Drug
  • Female
  • Gene Expression Regulation / drug effects
  • Oviposition / drug effects*
  • Receptors, Odorant / metabolism*
  • Skatole / pharmacology*

Substances

  • Receptors, Odorant
  • Skatole