Effect of NRG1, GDNF, EGF and NGF in the migration of a Schwann cell precursor line

Neurochem Res. 2010 Oct;35(10):1643-51. doi: 10.1007/s11064-010-0225-0. Epub 2010 Jul 13.

Abstract

The Schwann cells are the myelinating glia of the peripheral nervous system that originated during development from the highly motile neural crest. However, we do not know what the guidance signals for the Schwann cell precursors are. Therefore, we set to test some of the known neurotrophins that are expressed early in developing embryos and have been shown to be critical for the survival and patterning of developing glia and neurons. The goal of this study was to determine more specifically if GDNF, NRG1 and NGF are chemoattractants and/or chemokinetic molecules for a Schwann cell precursor line, the Spl201. We performed live chemoattraction assays, with imaging and also presented these molecules as part of their growing substrate. Our results show for the first time that GDNF and NRG1 are potent chemoattractive and chemokinetic molecules for these cells while NGF is a chemokinetic molecule stimulating their motility.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Chemotactic Factors / physiology*
  • Chemotaxis
  • Epidermal Growth Factor / physiology*
  • Glial Cell Line-Derived Neurotrophic Factor / physiology*
  • Nerve Growth Factor / physiology*
  • Neural Stem Cells / physiology*
  • Neuregulin-1 / physiology*
  • Rats
  • Schwann Cells / physiology*

Substances

  • Chemotactic Factors
  • Glial Cell Line-Derived Neurotrophic Factor
  • Neuregulin-1
  • Nrg1 protein, rat
  • Epidermal Growth Factor
  • Nerve Growth Factor