Modules for C-terminal epitope tagging of Tetrahymena genes

J Microbiol Methods. 2010 Sep;82(3):342-6. doi: 10.1016/j.mimet.2010.07.009. Epub 2010 Jul 17.

Abstract

Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Base Sequence
  • Genetic Techniques*
  • Molecular Sequence Data
  • Plasmids / genetics
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / genetics
  • Tetrahymena / chemistry
  • Tetrahymena / genetics*

Substances

  • Protozoan Proteins