Two hypervariable minisatellite DNA binding proteins

Nucleic Acids Res. 1991 Jun 25;19(12):3269-74. doi: 10.1093/nar/19.12.3269.


Hypervariable minisatellite DNA sequences are short, tandemly repeated sequences present at numerous loci in eukaryotes. They stimulate intermolecular homologous recombination up to 13-fold in human cells in culture and may be specific sites for the initiation of recombination in the eukaryotic genome (Wahls, W.P., Wallace, L.J., & Moore, P.D. (1990) Cell 60, 95-103). Reported here is the detection and partial purification of two hypervariable minisatellite DNA binding proteins, called Msbp-2 and Msbp-3, present in the nuclear extracts of human HeLa cells. The proteins elute from a gel filtration column with a native mass of 200-250 kDa and have sizes of 77 kDa and 115 kDa respectively.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • DNA
  • DNA, Satellite / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization


  • DNA, Satellite
  • DNA-Binding Proteins
  • DNA