O-antigen Diversity and Lateral Transfer of the Wbe Region Among Vibrio Splendidus Isolates

Environ Microbiol. 2010 Nov;12(11):2977-87. doi: 10.1111/j.1462-2920.2010.02274.x.

Abstract

The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antigenic Variation
  • Base Sequence
  • Gene Transfer, Horizontal*
  • Genetic Loci
  • Genetic Variation*
  • Genome, Bacterial
  • Massachusetts
  • Molecular Sequence Data
  • Multilocus Sequence Typing
  • O Antigens* / analysis
  • O Antigens* / classification
  • O Antigens* / genetics
  • Open Reading Frames
  • Phylogeny
  • Sequence Analysis, DNA
  • Serotyping
  • Vibrio / genetics*
  • Vibrio / immunology*
  • Water Microbiology

Substances

  • O Antigens

Associated data

  • GENBANK/GU990234
  • GENBANK/GU990235
  • GENBANK/GU990236
  • GENBANK/GU990237
  • GENBANK/GU990238
  • GENBANK/GU990239
  • GENBANK/GU990240
  • GENBANK/GU990241
  • GENBANK/GU990242
  • GENBANK/GU990243
  • GENBANK/GU990244
  • GENBANK/GU990245
  • GENBANK/GU990246
  • GENBANK/GU990247
  • GENBANK/GU990248
  • GENBANK/GU990249
  • GENBANK/GU990250
  • GENBANK/GU990251
  • GENBANK/GU990252
  • GENBANK/GU990253
  • GENBANK/GU990254
  • GENBANK/GU990255
  • GENBANK/GU990256
  • GENBANK/GU990257
  • GENBANK/GU990258
  • GENBANK/GU990259
  • GENBANK/GU990260
  • GENBANK/GU990261
  • GENBANK/GU990262
  • GENBANK/GU990263
  • GENBANK/GU990264
  • GENBANK/GU990265
  • GENBANK/GU990266
  • GENBANK/GU990267
  • GENBANK/GU990268
  • GENBANK/GU990269
  • GENBANK/GU990270
  • GENBANK/GU990271
  • GENBANK/GU990272
  • GENBANK/GU990273
  • GENBANK/GU990274
  • GENBANK/GU990275
  • GENBANK/GU990276
  • GENBANK/GU990277
  • GENBANK/GU990278
  • GENBANK/GU990279
  • GENBANK/GU990280
  • GENBANK/GU990281
  • GENBANK/GU990282
  • GENBANK/GU990283
  • GENBANK/GU990284
  • GENBANK/GU990285
  • GENBANK/GU990286
  • GENBANK/GU990287
  • GENBANK/GU990288
  • GENBANK/GU990289
  • GENBANK/GU990290
  • GENBANK/GU990291
  • GENBANK/GU990292
  • GENBANK/GU990293
  • GENBANK/GU990294
  • GENBANK/GU990295
  • GENBANK/GU990296
  • GENBANK/GU990297
  • GENBANK/GU990298
  • GENBANK/GU990299
  • GENBANK/GU990300
  • GENBANK/GU990301
  • GENBANK/GU990302
  • GENBANK/GU990303
  • GENBANK/GU990304
  • GENBANK/GU990305
  • GENBANK/GU990306
  • GENBANK/GU990307
  • GENBANK/GU990308
  • GENBANK/GU990309
  • GENBANK/GU990310
  • GENBANK/GU990311
  • GENBANK/GU990312
  • GENBANK/GU990313
  • GENBANK/GU990314
  • GENBANK/GU990315
  • GENBANK/GU990316
  • GENBANK/GU990317
  • GENBANK/GU990318
  • GENBANK/GU990319
  • GENBANK/GU990320
  • GENBANK/GU990321
  • GENBANK/GU990322
  • GENBANK/GU990323
  • GENBANK/GU990324
  • GENBANK/GU990325
  • GENBANK/GU990326
  • GENBANK/GU990327
  • GENBANK/GU990328
  • GENBANK/GU990329
  • GENBANK/GU990330
  • GENBANK/GU990331
  • GENBANK/GU990332
  • GENBANK/GU990333
  • GENBANK/GU990334
  • GENBANK/GU990335
  • GENBANK/GU990336
  • GENBANK/GU990337
  • GENBANK/GU990338
  • GENBANK/GU990339
  • GENBANK/GU990340
  • GENBANK/GU990341
  • GENBANK/GU990342
  • GENBANK/GU990343
  • GENBANK/GU990344
  • GENBANK/GU990345
  • GENBANK/GU990346
  • GENBANK/GU990347
  • GENBANK/GU990348
  • GENBANK/GU990349
  • GENBANK/GU990350
  • GENBANK/GU990351