The effect of Saccharomyces boulardii on Candida albicans-infected human intestinal cell lines Caco-2 and Intestin 407

FEMS Microbiol Lett. 2010 Sep 1;310(1):17-23. doi: 10.1111/j.1574-6968.2010.02037.x. Epub 2010 Jun 16.


Saccharomyces boulardii is a probiotic strain that confers many benefits to human enterocolopathies and is used against a number of enteric pathogens. Candida albicans is an opportunistic pathogen that causes intestinal infections in immunocompromised patients, and after translocation into the bloodstream, is responsible for serious systemic candidiasis. In this study, we investigated the influence of S. boulardii cells and its culture extract on C. albicans adhesion to Caco-2 and Intestin 407 cell lines. We also tested the proinflammatory IL-1beta, IL-6 and IL-8 cytokine expression by C. albicans-infected Caco-2 cells, using real-time RT-PCR. We found that both S. boulardii and its extract significantly inhibited C. albicans adhesion to epithelial cell lines. The IL-8 gene expression by C. albicans-infected Caco-2 cells was suppressed by the addition of S. boulardii extract. Our results indicate that S. boulardii affects C. albicans adhesion and reduces cytokine-mediated inflammatory host response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / growth & development
  • Candida albicans / pathogenicity*
  • Cell Adhesion
  • Cell Line
  • Epithelial Cells / microbiology*
  • Gene Expression Profiling
  • Humans
  • Interleukin-1beta / biosynthesis
  • Interleukin-6 / biosynthesis
  • Interleukin-8 / biosynthesis
  • Probiotics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces / growth & development
  • Saccharomyces / physiology*


  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8