Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans

J Clin Microbiol. 2010 Sep;48(9):3350-2. doi: 10.1128/JCM.00697-10. Epub 2010 Jul 14.

Abstract

We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Feces / parasitology*
  • Humans
  • Nucleic Acid Amplification Techniques / methods*
  • Parasitology / methods*
  • Sensitivity and Specificity
  • Taenia / classification*
  • Taenia / genetics
  • Taenia / isolation & purification*
  • Taeniasis / diagnosis*
  • Taeniasis / parasitology*