Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator's production and human cancer cell proliferation

J Ethnopharmacol. 2010 Sep 15;131(2):363-7. doi: 10.1016/j.jep.2010.07.020. Epub 2010 Jul 13.

Abstract

Aim of the study: The purpose of this study is to isolate the pure compounds from the extracts of Cordyceps militaris obtained through solid-state cultivation process, and evaluate their anti-inflammatory and anticancer properties.

Materials and methods: Silica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of 10 pure compounds (1-10). The compounds 1-10 were examined for their growth inhibitory properties against nitric oxide (NO), tumor necrosis factor (TNF)-alpha and interleukin (IL)-12 enhanced production from LPS/IFN-gamma-stimulated macrophages. Additionally, the anti-proliferation effects of 1-10 on human cancer cell lines, colon (colon 205), prostate (PC-3), and hepatoma (HepG2) cells were also analyzed.

Results: Compound 8 displayed potent growth inhibition on NO, TNF-alpha and IL-12 production with an IC(50) value of 7.5, 6.3, and 7.6 microg/ml, respectively. A similar inhibitory trend on these inflammatory mediators was observed for 3, 7, 9 and 10 with an IC(50) values ranging from 10.8 to 17.2 microg/ml. On the other hand, compounds 3 and 8 were potent anti-proliferative agents with an IC(50) value of 35.6 and 32.6 microg/ml toward PC-3 and colon 205 cell lines, respectively. The compounds 1 and 2 showed potent anti-proliferation in PC-3 and colon 205 cells, while only 3 displayed such effect in HepG2 cells.

Conclusion: The present study provides scientific supporting information for the ethnopharmacological use of Cordyceps militaris as an anti-inflammatory and anticancer agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use*
  • Biological Products / isolation & purification
  • Biological Products / pharmacology
  • Biological Products / therapeutic use*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Cordyceps / chemistry*
  • Humans
  • Inflammation Mediators / antagonists & inhibitors*
  • Inhibitory Concentration 50
  • Interferon-gamma
  • Interleukin-12 / antagonists & inhibitors
  • Lipopolysaccharides
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Neoplasms / drug therapy*
  • Neoplasms / metabolism
  • Nitric Oxide / antagonists & inhibitors
  • Phytotherapy
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors

Substances

  • Anti-Inflammatory Agents
  • Antineoplastic Agents
  • Biological Products
  • Inflammation Mediators
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Interleukin-12
  • Nitric Oxide
  • Interferon-gamma