Propolis prevents aluminium-induced genetic and hepatic damages in rat liver

Food Chem Toxicol. 2010 Oct;48(10):2741-6. doi: 10.1016/j.fct.2010.06.049. Epub 2010 Jul 14.

Abstract

Aluminium is present in several manufactured foods and medicines and is also used in water purification. Therefore, the present experiment was undertaken to determine the effectiveness of propolis in modulating the aluminium chloride (AlCl(3)) induced genotoxicity and hepatotoxicity in liver of rats. Animals were assigned to 1 of 4 groups: control; 34 mg AlCl(3)/kg bw; 50mg propolis/kg bw; AlCl(3) (34 mg/kg bw) plus propolis (50mg/kg bw), respectively. Rats were orally administered their respective doses daily for 30 days. At the end of the experiment, rats were anesthetized and hepatocytes (HEP) were isolated for counting the number of micronucleated hepatocytes (MNHEPs). In addition, the levels of serum enzymes and histological alterations in liver were investigated. AlCl(3) caused a significant increase in MNHEPs, alkaline phosphatase, transaminases (AST and ALT) and lactate dehydrogenase (LDH). Furthermore, severe pathological damages such as: sinusoidal dilatation, congestion of central vein, lipid accumulation and lymphocyte infiltration were established in liver. On the contrary, treatment with propolis alone did not cause any adverse effect on above parameters. Moreover, simultaneous treatments with propolis significantly modulated the toxic effects of AlCl(3). It can be concluded that propolis has beneficial influences and could be able to antagonize AlCl(3) toxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Alkaline Phosphatase / blood
  • Aluminum Chloride
  • Aluminum Compounds / antagonists & inhibitors*
  • Aluminum Compounds / toxicity*
  • Animals
  • Antimutagenic Agents*
  • Aspartate Aminotransferases / blood
  • Chemical and Drug Induced Liver Injury / enzymology
  • Chemical and Drug Induced Liver Injury / pathology
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Chlorides / antagonists & inhibitors*
  • Chlorides / toxicity*
  • Hepatocytes / drug effects
  • Hepatocytes / ultrastructure
  • L-Lactate Dehydrogenase / blood
  • Liver / enzymology
  • Liver / pathology
  • Liver Function Tests
  • Male
  • Micronucleus Tests
  • Oxidative Stress / drug effects
  • Propolis / pharmacology*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Aluminum Compounds
  • Antimutagenic Agents
  • Chlorides
  • Aluminum Chloride
  • Propolis
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Alkaline Phosphatase