Single-allele analysis of transcription kinetics in living mammalian cells

Nat Methods. 2010 Aug;7(8):631-3. doi: 10.1038/nmeth.1482. Epub 2010 Jul 18.


We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Cyclin D1 / genetics
  • DNA Replication
  • Humans
  • Kinetics
  • Transcription, Genetic*


  • Cyclin D1