High-throughput in vivo vertebrate screening

Nat Methods. 2010 Aug;7(8):634-6. doi: 10.1038/nmeth.1481. Epub 2010 Jul 18.


We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Automation
  • Axons*
  • Embryo, Nonmammalian
  • Equipment Design
  • Laser Therapy
  • Mass Screening / methods*
  • Microscopy, Confocal / instrumentation*
  • Models, Animal
  • Mutation
  • Retina / cytology*
  • Zebrafish