Amplification-free digital gene expression profiling from minute cell quantities

Nat Methods. 2010 Aug;7(8):619-21. doi: 10.1038/nmeth.1480. Epub 2010 Jul 18.


Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Gene Expression Profiling / methods*
  • Humans
  • Mice
  • Microarray Analysis / methods
  • Oligonucleotide Array Sequence Analysis / methods
  • Poly T / metabolism
  • RNA, Messenger / metabolism
  • Reverse Transcription
  • Sequence Analysis, DNA


  • RNA, Messenger
  • Poly T