Analgesic effect of Nigella sativa seeds extract on experimentally induced pain in albino mice

J Coll Physicians Surg Pak. 2010 Jul;20(7):464-7.


Objective: To determine the analgesic effect of ethanolic extract of Nigella sativa seeds on experimentally-induced pain in albino mice.

Study design: Randomized controlled trial (RCT).

Place and duration of study: Physiology Department, Services Institute of Medical Sciences (SIMS), Lahore, from May to September, 2009.

Methodology: The study was carried out in 90 male albino mice using acetic acid induced writhing test as a chemical model of nociception. The mice were divided in three groups of 30 each. Group A was given normal saline (control); group B was given Nigella sativa seed extract in a dose of 50 mg/kg; and group C received diclofenac sodium, as a reference drug. Number of writhings in treated and control groups were compared.

Results: The ethanolic extract of Nigella sativa seeds given intraperitoneally caused significant (p < 0.05) analgesic effect on nociceptive response initiated by 0.6% acetic acid; although this analgesic effect was less than that produced by diclofenac sodium.

Conclusion: Ethanolic extract of Nigella sativa possessed significant analgesic effect in mice.

MeSH terms

  • Analgesia / methods*
  • Animals
  • Male
  • Mice
  • Nigella sativa*
  • Pain / drug therapy*
  • Phytotherapy*
  • Plant Extracts / therapeutic use*
  • Seeds*


  • Plant Extracts