3'-5' tRNAHis guanylyltransferase in bacteria

FEBS Lett. 2010 Aug 20;584(16):3567-72. doi: 10.1016/j.febslet.2010.07.023. Epub 2010 Jul 23.

Abstract

The identity of the histidine specific transfer RNA (tRNA(His)) is largely determined by a unique guanosine residue at position -1. In eukaryotes and archaea, the tRNA(His) guanylyltransferase (Thg1) catalyzes 3'-5' addition of G to the 5'-terminus of tRNA(His). Here, we show that Thg1 also occurs in bacteria. We demonstrate in vitro Thg1 activity for recombinant enzymes from the two bacteria Bacillus thuringiensis and Myxococcus xanthus and provide a closer investigation of several archaeal Thg1. The reaction mechanism of prokaryotic Thg1 differs from eukaryotic enzymes, as it does not require ATP. Complementation of a yeast thg1 knockout strain with bacterial Thg1 verified in vivo activity and suggests a relaxed recognition of the discriminator base in bacteria.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anticodon / genetics
  • Anticodon / metabolism
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • Bacillus thuringiensis / enzymology
  • Bacillus thuringiensis / genetics
  • Bacteria / enzymology*
  • Bacteria / genetics
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Gene Knockout Techniques
  • Genes, Bacterial
  • Genes, Fungal
  • Genetic Complementation Test
  • Myxococcus xanthus / enzymology
  • Myxococcus xanthus / genetics
  • Nucleotidyltransferases / antagonists & inhibitors
  • Nucleotidyltransferases / genetics
  • Nucleotidyltransferases / metabolism*
  • Phylogeny
  • RNA, Transfer, His / genetics
  • RNA, Transfer, His / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / antagonists & inhibitors
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Species Specificity

Substances

  • Anticodon
  • Archaeal Proteins
  • Bacterial Proteins
  • RNA, Transfer, His
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Nucleotidyltransferases
  • Thg1 protein, S cerevisiae
  • tRNA guanylyltransferase