Surface integrity governs the proteome of hypomineralized enamel

J Dent Res. 2010 Oct;89(10):1160-5. doi: 10.1177/0022034510375824. Epub 2010 Jul 22.


Growing interest in the treatment and prevention of Molar/Incisor Hypomineralization (MIH) warrants investigation into the protein composition of hypomineralized enamel. Hypothesizing abnormality akin to amelogenesis imperfecta, we profiled proteins in hypomineralized enamel from human permanent first molars using a biochemical approach. Hypomineralized enamel was found to have from 3- to 15-fold higher protein content than normal, but a near-normal level of residual amelogenins. This distinguished MIH from hypomaturation defects with high residual amelogenins (amelogenesis imperfecta, fluorosis) and so typified it as a hypocalcification defect. Second, hypomineralized enamel was found to have accumulated various proteins from oral fluid and blood, with differential incorporation depending on integrity of the enamel surface. Pathogenically, these results point to a pre-eruptive disturbance of mineralization involving albumin and, in cases with post-eruptive breakdown, subsequent protein adsorption on the exposed hydroxyapatite matrix. These insights into the pathogenesis and properties of hypomineralized enamel hold significance for prevention and treatment of MIH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Amelogenin / analysis
  • Animals
  • Blood Proteins / analysis
  • Child
  • Complement C3 / analysis
  • Dental Enamel / chemistry
  • Dental Enamel / pathology
  • Dental Enamel Hypoplasia / metabolism
  • Dental Enamel Hypoplasia / pathology*
  • Dental Enamel Proteins / analysis*
  • Durapatite / analysis
  • Durapatite / metabolism
  • Hemoglobins / analysis
  • Hemoglobins / pharmacokinetics
  • Humans
  • Molar / chemistry
  • Molar / pathology
  • Peptide Fragments / analysis
  • Proteome / analysis*
  • Rats
  • Rats, Sprague-Dawley
  • Salivary Proteins and Peptides / analysis
  • Serum Albumin / analysis
  • Serum Albumin / pharmacokinetics
  • Tooth Calcification


  • Amelogenin
  • Blood Proteins
  • Complement C3
  • Dental Enamel Proteins
  • Hemoglobins
  • Peptide Fragments
  • Proteome
  • Salivary Proteins and Peptides
  • Serum Albumin
  • Durapatite