Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis

Curr Genet. 1991 Feb;19(2):109-18. doi: 10.1007/BF00326291.

Abstract

Linear hybrid plasmids based on the killer plasmid pGKL1 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae. Like pGKL1, the hybrids are located in the cytoplasm, have terminal inverted repeats (TIR) and possess covalently linked proteins at their 5' ends. The construction of cytoplasmic hybrid plasmids is based on the use of a pGKL1 promoter to control the marker gene used for recombination. Nuclear promoters are not recognised in the cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Fungal
  • Gene Expression*
  • Kluyveromyces / genetics*
  • Molecular Sequence Data
  • Phenotype
  • Plasmids*
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Restriction Mapping
  • Transcription, Genetic
  • Transformation, Genetic

Substances

  • DNA, Fungal