Structured triacylglycerol containing behenic and oleic acids suppresses triacylglycerol absorption and prevents obesity in rats

Lipids Health Dis. 2010 Jul 24;9:77. doi: 10.1186/1476-511X-9-77.

Abstract

Background: Dietary 1(3)-behenoyl-2,3(1)-dioleoyl-rac-glycerol (BOO) has been reported to inhibit pancreatic lipase activity in vitro and suppress postprandial hypertriacylglycerolemia in humans. In the present study, the anti-obesity activities of BOO and its inhibitory effects on lymphatic triacylglycerol (TAG) absorption were investigated in rats.

Methods: In Experiment 1, rats were fed either BOO or soybean oil (SO) diet for 6 weeks. In the BOO diet, 20% of SO was replaced with an experimental oil rich in BOO. In Experiments 2 and 3, rats cannulated in the thoracic duct were administered an emulsions containing trioleoylglycerol (OOO) or an oil mixture (OOO:BOO, 9:1). Tri[1-14C]oleoylglycerol (14C-OOO) was added to the emulsions administered in Experiment 3.

Results: No observable differences were detected in food intake or body weight gain between the BOO and SO groups in Experiment 1. Plasma and liver TAG concentrations and visceral fat weights were significantly lower in the BOO group than in the SO group. The apparent absorption rate of fat was significantly lower in the BOO group than in the SO group. In Experiment 2, the lymphatic recovery of oleic and behenic acids was significantly lower at 5 and 6 h after BOO administration than after OOO administration. In Experiment 3, the lymphatic recovery of 14C-OOO was significantly lower at 5 and 6 h after BOO administration than after OOO administration.

Conclusions: These results suggest that BOO prevents deposition of visceral fat and hepatic TAG by lowering and delaying intestinal absorption of TAG.

MeSH terms

  • Animals
  • Anti-Obesity Agents / chemical synthesis
  • Anti-Obesity Agents / therapeutic use*
  • Cholesterol / blood
  • Cholesterol / metabolism
  • Eating
  • Fat Substitutes / chemical synthesis
  • Fat Substitutes / therapeutic use
  • Fatty Acids* / metabolism
  • Feces / chemistry
  • Hypertriglyceridemia / blood
  • Hypertriglyceridemia / prevention & control
  • Intestinal Absorption*
  • Intra-Abdominal Fat / pathology
  • Kinetics
  • Liver / metabolism
  • Liver / pathology
  • Lymph / metabolism
  • Lymphatic System / physiology
  • Male
  • Obesity / blood
  • Obesity / diet therapy
  • Obesity / pathology
  • Obesity / prevention & control*
  • Oleic Acid* / metabolism
  • Organ Size
  • Rats
  • Rats, Wistar
  • Triglycerides / blood
  • Triglycerides / chemical synthesis
  • Triglycerides / metabolism*
  • Triglycerides / therapeutic use*
  • Weight Gain

Substances

  • Anti-Obesity Agents
  • Fat Substitutes
  • Fatty Acids
  • Triglycerides
  • Oleic Acid
  • Cholesterol
  • behenic acid