Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH(2), CH(3), and OH functions

Dent Mater J. 2010 Aug;29(4):438-45. doi: 10.4012/dmj.2010-017. Epub 2010 Jul 17.


Hydroxyapatite formation was examined at the surface of self-assembled monolayers (SAMs) modified with four functional groups, -COOH, -NH(2), -CH(3), and -OH. For COOH-SAM and NH(2)-SAM, scanning electron spectroscopic observation showed that flake-like sheet crystals covered the whole wafer and small broccoli-like crystals were observed occasionally on the flake-like crystal base layer. For CH(3)-SAM and OH-SAM, no flake-like sheet crystals were observed; broccoli-like crystals were observed in a dispersed manner for CH(3)-SAM, but in localized spots for OH-SAM. X-ray diffraction patterns showed a strong apatite pattern oriented toward the c-axis direction for COOH-SAM. ESCA analysis revealed distinct Ca, P, O peaks for COOH-, NH(2)-, CH(3)-, and OH-SAM. Surface plasmon resonance (SPR) analysis indicated that during the supply of supersaturated calcium phosphate solution, the deposition of precipitates increased monotonically with time for COOH-SAM, increased slightly for NH(2)-SAM, but little increase in deposition was detected for CH(3)-SAM and OH-SAM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apatites / analysis
  • Apatites / chemistry
  • Calcium Phosphates / analysis
  • Calcium Phosphates / chemistry*
  • Carboxylic Acids / analysis
  • Carboxylic Acids / chemistry*
  • Chemical Precipitation
  • Crystallography
  • Durapatite / analysis
  • Durapatite / chemistry*
  • Gold Alloys / chemistry
  • Humans
  • Hydroxides / analysis
  • Hydroxides / chemistry*
  • Membranes, Artificial*
  • Methane / analogs & derivatives*
  • Methane / analysis
  • Methane / chemistry
  • Microscopy, Electron, Scanning
  • Nitrogen / analysis
  • Nitrogen / chemistry*
  • Photoelectron Spectroscopy
  • Surface Plasmon Resonance
  • Wettability
  • X-Ray Diffraction


  • Apatites
  • Calcium Phosphates
  • Carboxylic Acids
  • Gold Alloys
  • Hydroxides
  • Membranes, Artificial
  • methyl radical
  • hydroxide ion
  • Durapatite
  • calcium phosphate
  • Nitrogen
  • Methane