Comparing different methods used to collect material for a microbiological evaluation of patients with chronic rhinosinusitis

Braz J Otorhinolaryngol. May-Jun 2010;76(3):321-5.


There is still controversy on which is the best method to collect the secretion directly from the middle meatus or maxillary sinus in patients with chronic rhinosinusitis.

Aim: To evaluate the prevalence of bacteria in patients with chronic rhinosinusitis and compare the suction trap collector to direct aspiration attached to a syringe for the microbiological analysis of these secretions.

Materials and methods: Prospective study involving 31 patients who underwent endoscopically guided maxillary secretion aspiration by two different methods (aspiration with the collector tube "suction trap" and aspiration with the use of a catheter connected to a syringe), to determine the microbiological diagnosis and to compare the two methods used.

Results: microorganisms grew samples collected from 55% of the 31 patients. The most frequent bacteria were S. aureus, Pseudomonas aeruginosa and other aerobic Gram-negative bacteria. The results from cultures were similar between the two methods in 71% of patients.

Conclusion: S. aureus, Pseudomonas aeruginosa and other aerobic Gram-negative bacteria make up the main flora in the maxillary sinus of the patients. There was good correlation between the microbiological results obtained by using a catheter attached to a syringe and the "suction trap" nasal collector.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Chronic Disease
  • Cross-Sectional Studies
  • Female
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Positive Bacteria / classification
  • Gram-Positive Bacteria / isolation & purification*
  • Humans
  • Male
  • Maxillary Sinusitis / microbiology*
  • Middle Aged
  • Prospective Studies
  • Pseudomonas aeruginosa / isolation & purification
  • Rhinitis / microbiology*
  • Specimen Handling / instrumentation
  • Specimen Handling / methods*
  • Staphylococcus aureus / isolation & purification
  • Young Adult