Effects of probiotic Lactobacillus salivarius WB21 on halitosis and oral health: an open-label pilot trial

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Aug;110(2):201-8. doi: 10.1016/j.tripleo.2010.03.032.


Objective: The objective of this study was to evaluate whether oral administration of lactobacilli alters the degree of halitosis and clinical conditions associated with halitosis.

Study design: Twenty patients with genuine halitosis were given 2.0 x 10(9)Lactobacillus salivarius WB21 and xylitol in tablet form daily. Oral malodor and clinical parameters were evaluated at the same time of day for each patient after 2 and 4 weeks.

Results: All 20 patients were positive for L. salivarius DNA in their saliva at 2 weeks, although 12 patients were negative for this organism at baseline. Oral malodor parameters significantly decreased at 2 weeks in the subjects with physiologic halitosis. The scores of an organoleptic test and bleeding on probing significantly decreased at 4 weeks in the subjects with oral pathologic halitosis.

Conclusions: Oral administration of probiotic lactobacilli primarily improved physiologic halitosis and also showed beneficial effects on bleeding on probing from the periodontal pocket.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Breath Tests
  • Chi-Square Distribution
  • Colony Count, Microbial
  • DNA, Bacterial / analysis
  • Dental Plaque Index
  • Female
  • Halitosis / therapy*
  • Humans
  • Lactobacillus*
  • Logistic Models
  • Male
  • Middle Aged
  • Periodontal Pocket / therapy*
  • Pilot Projects
  • Porphyromonas gingivalis / isolation & purification
  • Prevotella intermedia / isolation & purification
  • Probiotics / therapeutic use*
  • Statistics, Nonparametric
  • Streptococcus mutans / isolation & purification
  • Streptococcus sobrinus / isolation & purification
  • Sulfur Compounds / analysis


  • DNA, Bacterial
  • Sulfur Compounds