Targeting DNA double-strand breaks with TAL effector nucleases

Genetics. 2010 Oct;186(2):757-61. doi: 10.1534/genetics.110.120717. Epub 2010 Jul 26.

Abstract

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites / genetics
  • Catalytic Domain / genetics
  • DNA / genetics
  • DNA Breaks, Double-Stranded*
  • DNA Repair
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism
  • Deoxyribonucleases, Type II Site-Specific / chemistry
  • Deoxyribonucleases, Type II Site-Specific / genetics*
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Gene Targeting*
  • Genetic Engineering*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • Transcriptional Activation
  • Xanthomonas
  • Xanthomonas campestris
  • Zinc Fingers / genetics

Substances

  • DNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors
  • DNA
  • endodeoxyribonuclease FokI
  • Deoxyribonucleases, Type II Site-Specific