Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis

Mol Vis. 2010 Jul 17;16:1382-8.

Abstract

Purpose: To investigate the effect of eicosapentaenoic acid (EPA) on acute ocular inflammation in an animal model of endotoxin-induced uveitis (EIU).

Methods: C57Bl/6 mice (6-week-old males) were orally treated with EPA at a dose of 50 mg/kg/day for 5 days. EIU was then induced in the animals by intraperitoneal injection of 160 microg lipopolysaccharide (LPS). Twenty-four hours after LPS injection, leukocyte adhesion to the retinal vasculature was evaluated by the concanavalin A lectin perfusion-labeling technique, and leukocyte infiltration into the vitreous cavity was quantified. Furthermore, the protein levels of monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, intercellular adhesion molecule-1 and phospholyrated nuclear factor (NF)-kappaB p65 in the retina and retinal pigment epithelium (RPE)-choroid complex were examined by enzyme-linked immunosorbent assay (ELISA).

Results: At 24 h after LPS injection, the EIU animals treated with oral EPA administration showed a significant decrease in leukocyte adhesion to the retinal vessels by 43.4% (p<0.01) and leukocyte infiltration into the vitreous cavity by 49.2% (p<0.05). In addition, EPA significantly reduced the protein levels of MCP-1 and IL-6 in the retina and the RPE-choroid complex. Furthermore, phosphorylation of NF-kappaB was suppressed by EPA treatment.

Conclusions: Our data suggest that EPA inhibits multiple inflammatory molecules in vivo. EPA may become a novel strategy in the prevention and/or treatment of ocular inflammatory diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules / metabolism
  • Cell Movement / drug effects
  • Choroid / drug effects
  • Choroid / metabolism
  • Choroid / pathology
  • Eicosapentaenoic Acid / pharmacology*
  • Endotoxins / pharmacology*
  • Inflammation / chemically induced
  • Inflammation / complications*
  • Inflammation / prevention & control*
  • Inflammation Mediators / metabolism
  • Leukocytes / drug effects
  • Leukocytes / pathology
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B / metabolism
  • Retinal Pigment Epithelium / drug effects
  • Retinal Pigment Epithelium / metabolism
  • Retinal Pigment Epithelium / pathology
  • Retinal Vessels / drug effects
  • Retinal Vessels / metabolism
  • Retinal Vessels / pathology
  • Uveitis / chemically induced
  • Uveitis / complications*
  • Uveitis / prevention & control*

Substances

  • Cell Adhesion Molecules
  • Endotoxins
  • Inflammation Mediators
  • NF-kappa B
  • Eicosapentaenoic Acid