Abstract
In our previous study, lancemaside A isolated from Codonopsis lanceolata (family Campanulaceae) ameliorated colitis in mice. In this study, the anti-inflammatory effects of lancemaside A was investigated in lipopolysaccharide (LPS)-stimulated mice and their peritoneal macrophage cells. Lancemaside A suppressed the production of pro-inflammatory cytokines, TNF-α and IL-1β, in vitro and in vivo. Lancemaside A also down-regulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as the inflammatory mediators, nitric oxide (NO), and PGE(2). Lancemaside A also inhibited the expression of IL-1 receptor-associated kinase-4 (IRAK-4), the phosphorylation of IKK-β and IκB-α, the nuclear translocation of NF-κB and the activation of mitogen-activated protein kinases in LPS-stimulated peritoneal macrophages. Furthermore, lancemaisde A inhibited the interaction between LPS and TLR4, as well as IRAK-4 expression in peritoneal macrophages. Based on these findings, lancemaside A expressed anti-inflammatory effects by regulating both the binding of LPS to TLR4 on macrophages.
© 2010 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cyclooxygenase 2 / biosynthesis
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Cytokines / biosynthesis
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I-kappa B Kinase / metabolism
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I-kappa B Proteins / metabolism
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Inflammation / drug therapy*
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Inflammation / immunology
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Inflammation / pathology
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Inflammation Mediators / metabolism
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Lipopolysaccharides / metabolism*
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Macrophage Activation / drug effects
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / enzymology
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Macrophages, Peritoneal / immunology
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Macrophages, Peritoneal / pathology
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Male
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Mice
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Mice, Inbred ICR
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NF-KappaB Inhibitor alpha
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NF-kappa B / metabolism
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Nitric Oxide Synthase Type II / biosynthesis
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Phosphorylation / drug effects
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Protein Binding / drug effects
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Protein Processing, Post-Translational / drug effects
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Saponins / chemistry
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Saponins / pharmacology
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Saponins / therapeutic use*
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Toll-Like Receptor 4 / metabolism*
Substances
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Cytokines
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I-kappa B Proteins
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Inflammation Mediators
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Lipopolysaccharides
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NF-kappa B
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Nfkbia protein, mouse
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Saponins
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Tlr4 protein, mouse
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Toll-Like Receptor 4
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lancemaside A
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NF-KappaB Inhibitor alpha
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Nitric Oxide Synthase Type II
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Ptgs2 protein, mouse
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Cyclooxygenase 2
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I-kappa B Kinase