Stable gold nanoparticle conjugation to internal DNA positions: facile generation of discrete gold nanoparticle-DNA assemblies

Bioconjug Chem. 2010 Aug 18;21(8):1413-6. doi: 10.1021/bc100160k.


A straightforward strategy to stably anchor one or more gold nanoparticles (AuNPs) at both the internal and terminal positions of single-stranded DNA is presented. Discrete DNA-AuNP conjugates are isolated using a noncovalent extension strand strategy, that helps to resolve prepared species by agarose gel electrophoresis (AGE). These are used to assemble well-defined AuNP squares and rectangles. Two complementary bis-AuNP-labeled DNA conjugates are then prepared. One of these places two smaller (5 nm) AuNPs at defined internal positions within a DNA strand, and the other places two larger (13 nm) AuNPs at each of its terminal positions. We show the self-assembly of these bis-AuNP conjugates into a tapered tetrameric gold nanoparticle "antenna" structures of direct relevance to engineered "hot spots" and surface enhanced Raman scattering (SERS) substrates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • DNA / chemistry*
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Particle Size
  • Spectrum Analysis, Raman
  • Sulfhydryl Compounds / chemistry
  • Surface Properties


  • Sulfhydryl Compounds
  • Gold
  • DNA