Cloning and in vitro characterization of dTDP-6-deoxy-L-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-L-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-L-talose

Carbohydr Res. 2010 Sep 3;345(13):1958-62. doi: 10.1016/j.carres.2010.07.004.

Abstract

Kitasatospora kifunensis, the talosin producer, was used as a source for the dTDP-6-deoxy-l-talose (dTDP-6dTal) biosynthetic gene cluster, serving as a template for four recombinant proteins of RmlA(Kkf), RmlB(Kkf), RmlC(Kkf), and Tal, which complete the biosynthesis of dTDP-6dTal from dTTP, alpha-d-glucose-1-phosphate, and NAD(P)H. The identity of dTDP-6dTal was validated using (1)H and (13)C NMR spectroscopy. K. kifunensistal and tll, the known dTDP-6dTal synthase gene of Actinobacillus actinomycetemcomitans origin, have low sequence similarity and are distantly related within the NDP-6-deoxy-4-ketohexose reductase family, providing an example of the genetic diversity within the dTDP-6dTal biosynthetic pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Genetic Variation
  • Lactones / metabolism*
  • Molecular Sequence Data
  • Nucleoside Diphosphate Sugars / metabolism*
  • Streptomycetaceae / enzymology
  • Streptomycetaceae / genetics*
  • Streptomycetaceae / metabolism*
  • Sugar Alcohol Dehydrogenases / genetics*
  • Sugar Alcohol Dehydrogenases / isolation & purification
  • Sugar Alcohol Dehydrogenases / metabolism*

Substances

  • L-talo-gamma-lactone
  • Lactones
  • Nucleoside Diphosphate Sugars
  • dTDP-6-deoxytalose
  • Sugar Alcohol Dehydrogenases
  • thymidine diphosphate-6-deoxy-L-lyxo-4-hexulose reductase

Associated data

  • GENBANK/HM132058