Detection of Classical and Newly Described Staphylococcal Superantigen Genes in Coagulase-Negative Staphylococci Isolated From Bovine Intramammary Infections

Vet Microbiol. 2011 Jan 10;147(1-2):149-54. doi: 10.1016/j.vetmic.2010.06.021. Epub 2010 Jul 1.

Abstract

The coagulase-negative staphylococci (CNS) are the most prevalent mastitis pathogen group yet their virulence characteristics have not been well described. We investigated the presence of 19 classical and newly described staphylococcal superantigen (SAg) genes in CNS isolates from bovine intramammary infections (IMI). A total of 263 CNS representing 11 different Staphylococcus spp. were examined, and 31.2% (n=82) of CNS isolates had one or more SAg genes; there were 21 different SAg gene combinations. The most prevalent combination of SAg genes (seb, seln and selq; n=45) was found in S. chromogenes, S. xylosus, S. haemolyticus, S. sciuri subsp. carnaticus, S. simulans and S. succinus. The genes for SAgs appear to be widely distributed amongst CNS isolated from bovine IMI.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Coagulase / metabolism
  • Female
  • Mastitis, Bovine / microbiology*
  • Polymerase Chain Reaction / veterinary
  • Prevalence
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary*
  • Staphylococcus / enzymology
  • Staphylococcus / genetics*
  • Superantigens / genetics*

Substances

  • Coagulase
  • Superantigens