PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica

Mol Cell. 2010 Jul 30;39(2):209-21. doi: 10.1016/j.molcel.2010.06.021.


We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Drug Resistance, Microbial*
  • Female
  • Gene Expression Regulation, Bacterial / genetics
  • Genomic Islands / genetics
  • Lysine / analogs & derivatives
  • Lysine / genetics
  • Lysine / metabolism
  • Lysine-tRNA Ligase / genetics
  • Lysine-tRNA Ligase / metabolism
  • Mice
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism*
  • Protein Processing, Post-Translational*
  • Salmonella enterica* / metabolism
  • Salmonella enterica* / pathogenicity
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*


  • Bacterial Proteins
  • Peptide Elongation Factors
  • Virulence Factors
  • factor EF-P
  • hypusine
  • Lysine-tRNA Ligase
  • Lysine

Associated data

  • PDB/3G1Z