Polysaccharide purified from Polyporus umbellatus (Per) Fr induces the activation and maturation of murine bone-derived dendritic cells via toll-like receptor 4

Cell Immunol. 2010;265(1):50-6. doi: 10.1016/j.cellimm.2010.07.002. Epub 2010 Jul 14.


In this study, we report that a polysaccharide isolated from a Chinese medicinal herb, Zhu Ling (the sclerotium of Polyporus umbellatus (Per) Fr), induces phenotypic and functional maturation of murine bone-derived dendritic cells (BMDCs). Treatment of BMDCs with Polyporus polysaccharide (PPS) resulted in enhanced cell-surface expression of CD86, as well as enhanced production of both interleukin (IL)-12 p40 and IL-10 in a dose-dependent manner. In addition, treatment of BMDCs with PPS resulted in increased T cell-stimulatory capacity and decreased phagocytic ability. PPS-induced production of IL-12 p40 was inhibited by monoclonal antibodies to Toll-like receptor 4 (TLR4). Flow cytometric analysis showed that fluorescence-labeled PPS (f-PPS) bound specifically to BMDCs. This binding was blocked by both unlabeled PPS and anti-TLR4, but not by anti-TLR2 and anti-CR3 monoclonal antibodies. Taken together, our data show that PPS promotes the activation and maturation of murine BMDCs via TLR4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / immunology*
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology*
  • Dendritic Cells / immunology*
  • Drugs, Chinese Herbal / pharmacology
  • Female
  • Interleukin-10 / immunology
  • Interleukin-12 Subunit p40 / immunology
  • Lymphocyte Culture Test, Mixed
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Polyporus / chemistry
  • Polyporus / immunology*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Toll-Like Receptor 4 / immunology*


  • Drugs, Chinese Herbal
  • Interleukin-12 Subunit p40
  • Polysaccharides
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • Interleukin-10