Enhanced production of a plant monoterpene by overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A reductase catalytic domain in Saccharomyces cerevisiae

Appl Environ Microbiol. 2010 Oct;76(19):6449-54. doi: 10.1128/AEM.02987-09. Epub 2010 Jul 30.

Abstract

Linalool production was evaluated in different Saccharomyces cerevisiae strains expressing the Clarkia breweri linalool synthase gene (LIS). The wine strain T(73) was shown to produce higher levels of linalool than conventional laboratory strains (i.e., almost three times the amount). The performance of this strain was further enhanced by manipulating the endogenous mevalonate (MVA) pathway: deregulated overexpression of the rate-limiting 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) doubled linalool production. In a haploid laboratory strain, engineering of this key step also improved linalool yield.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclic Monoterpenes
  • Biotechnology / methods*
  • Catalytic Domain / genetics
  • Clarkia / enzymology
  • Clarkia / genetics
  • Gene Expression*
  • Genetic Engineering
  • Hydro-Lyases / genetics
  • Hydro-Lyases / metabolism*
  • Hydroxymethylglutaryl CoA Reductases / genetics
  • Hydroxymethylglutaryl CoA Reductases / metabolism*
  • Metabolic Networks and Pathways / genetics
  • Mevalonic Acid / metabolism
  • Monoterpenes / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Acyclic Monoterpenes
  • Monoterpenes
  • Recombinant Proteins
  • linalool
  • Hydroxymethylglutaryl CoA Reductases
  • Hydro-Lyases
  • S-linalool synthase
  • Mevalonic Acid