Mechanical regulation of cell function with geometrically modulated elastomeric substrates

Nat Methods. 2010 Sep;7(9):733-6. doi: 10.1038/nmeth.1487. Epub 2010 Aug 1.


We report the establishment of a library of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrated that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility and stem cell differentiation. Furthermore, early changes in cytoskeletal contractility predicted later stem cell fate decisions in single cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Adhesion / physiology
  • Cells, Cultured
  • Dimethylpolysiloxanes / chemistry
  • Elastomers
  • Endothelial Cells / cytology*
  • Humans
  • Materials Testing
  • Mesenchymal Stem Cells / cytology*
  • Microscopy, Atomic Force / instrumentation
  • Particle Size
  • Polymers / chemistry*
  • Silicon / chemistry
  • Stress, Mechanical*
  • Surface Properties


  • Dimethylpolysiloxanes
  • Elastomers
  • Polymers
  • elastomeric polymer
  • baysilon
  • Silicon