Is repeat PCR needed for diagnosis of Clostridium difficile infection?

J Clin Microbiol. 2010 Oct;48(10):3738-41. doi: 10.1128/JCM.00722-10. Epub 2010 Aug 4.

Abstract

Patients with diarrhea, defined as loose or watery stool, and two or more Clostridium difficile tcdB PCR tests within 14 days of each other were investigated. Repeat PCR for 293 patients with a prior negative result yielded negative results in 396 (97.5%) of 406 tests. Ten new positives were detected, including one false positive. Repeat PCR within 7 days appears rarely useful, except for patients with evidence of a new infection.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacterial Proteins / genetics
  • Bacterial Toxins / genetics
  • Bacteriological Techniques / methods*
  • Child
  • Child, Preschool
  • Clostridium Infections / diagnosis*
  • Clostridium Infections / microbiology*
  • Clostridium difficile / genetics
  • Clostridium difficile / isolation & purification*
  • Diarrhea / microbiology
  • Humans
  • Infant
  • Middle Aged
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Young Adult

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • toxB protein, Clostridium difficile