Molecular Analysis of Cryptosporidium Parvum HNJ-1 Isolated in Japan

J Vet Med Sci. 2010 Dec;72(12):1647-9. doi: 10.1292/jvms.10-0118. Epub 2010 Aug 3.

Abstract

Cryptosporidium parvum HNJ-1 is widely used as a reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on the sequence results of GP60 gene locus.

MeSH terms

  • Animals
  • Cryptosporidiosis / epidemiology
  • Cryptosporidium parvum / classification*
  • Cryptosporidium parvum / genetics
  • Cryptosporidium parvum / isolation & purification*
  • DNA, Protozoan / genetics
  • DNA, Ribosomal Spacer / genetics
  • Gene Expression Regulation
  • Glycoproteins / genetics
  • Humans
  • Japan / epidemiology
  • Mice
  • Phylogeny
  • Polymorphism, Genetic
  • RNA, Protozoan / genetics
  • Tetrahydrofolate Dehydrogenase / genetics

Substances

  • DNA, Protozoan
  • DNA, Ribosomal Spacer
  • Glycoproteins
  • RNA, Protozoan
  • Tetrahydrofolate Dehydrogenase