A biochemical investigation of auranofin nephrotoxicity by high field proton nuclear magnetic resonance (NMR) spectroscopy

Agents Actions Suppl. 1991;32:59-63. doi: 10.1007/978-3-0348-7405-2_7.

Abstract

High-field proton NMR spectroscopic analysis of urine and plasma has been employed to study the biochemical effects and nephrotoxic action of an intramuscular dose of auranofin in rats. Auranofin induced a characteristic profile of proximal tubular damage as evidenced by aminoaciduria, lactic aciduria and increased urinary acetate concentrations. In addition, ethanol was detectable in both urine and plasma obtained from auranofin-treated rats. Auranofin-mediated elevations in the plasma and urine concentrations of 3-D-hydroxybutyrate indicated an increased utilisation of fats for fuel in rats treated with this novel therapeutic agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / blood
  • Alcohol Dehydrogenase / antagonists & inhibitors
  • Amino Acids / urine
  • Animals
  • Auranofin*
  • Hydroxybutyrates / blood
  • Ketoglutaric Acids / blood
  • Kidney Diseases / chemically induced*
  • Kidney Diseases / metabolism
  • Magnetic Resonance Spectroscopy
  • Male
  • Oxidation-Reduction
  • Rats
  • Rats, Inbred Strains

Substances

  • Acetates
  • Amino Acids
  • Hydroxybutyrates
  • Ketoglutaric Acids
  • Auranofin
  • Alcohol Dehydrogenase