Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice

BMC Dev Biol. 2010 Aug 6;10:84. doi: 10.1186/1471-213X-10-84.


Background: Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear.

Results: We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos.

Conclusions: Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyonic tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Vessels / metabolism
  • Embryo Implantation
  • Embryo, Mammalian / blood supply*
  • Female
  • GPI-Linked Proteins
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Neovascularization, Physiologic*
  • Pregnancy
  • Uterus / blood supply*


  • GPI-Linked Proteins
  • Membrane Glycoproteins
  • Reck protein, mouse