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, 7 (9), 729-32

Spontaneous Network Activity Visualized by Ultrasensitive Ca(2+) Indicators, Yellow Cameleon-Nano

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Spontaneous Network Activity Visualized by Ultrasensitive Ca(2+) Indicators, Yellow Cameleon-Nano

Kazuki Horikawa et al. Nat Methods.

Abstract

We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.

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References

    1. Nat Methods. 2008 Sep;5(9):805-11 - PubMed
    1. Am J Physiol. 1982 May;242(5):C404-8 - PubMed
    1. Nat Methods. 2008 Sep;5(9):797-804 - PubMed
    1. Biochemistry. 1996 Mar 19;35(11):3508-17 - PubMed
    1. Methods Enzymol. 1989;172:230-62 - PubMed
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