Spontaneous Network Activity Visualized by Ultrasensitive Ca(2+) Indicators, Yellow Cameleon-Nano

Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 2010 Aug 8.

Abstract

We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.

MeSH terms

  • Animals
  • Calcium / analysis*
  • Calcium / metabolism
  • Dictyostelium
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / chemistry
  • Indicators and Reagents / analysis
  • Indicators and Reagents / chemistry
  • Mice
  • Molecular Sequence Data
  • Neurons / metabolism
  • Signal Transduction
  • Zebrafish

Substances

  • Fluorescent Dyes
  • Indicators and Reagents
  • Calcium

Associated data

  • GENBANK/GU071083
  • GENBANK/GU071084
  • GENBANK/GU071085
  • GENBANK/HM145948
  • GENBANK/HM145949