Transgenic RNAi applications in the mouse

Methods Enzymol. 2010:477:367-86. doi: 10.1016/S0076-6879(10)77019-1.

Abstract

Within the past 10 years, RNA interference has emerged as a powerful experimental tool as it allows rapid gene function analysis. Unique features such as reversibility of gene silencing and simultaneous targeting of several genes characterize the approach. In this chapter, transgenic RNAi techniques in reverse mouse genetics are discussed and protocols are provided.

MeSH terms

  • Animals
  • Gene Silencing / physiology*
  • Mice
  • Mice, Transgenic
  • RNA Interference / physiology*